Histone H1T Antibody - BSA Free

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human testis shows nuclear positivity in ductus seminiferous.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human cerebral cortex, colon, kidney and testis using Anti-HIST1H1T antibody NBP2-56042 (A) shows similar protein distribution across tissues to independent antibody NBP2-57668 (B).

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human kidney.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP2-56042] - Staining of human colon.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H1T Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RATTPKTVRSGRKAKGAKGKQQQKSPVKARASKSKLTQHHEVNVRKATSKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H1-6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Histone H1T Recombinant Protein Antigen (NBP2-56042PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H1T Antibody - BSA Free

• dJ221C16.2
• H1 histone family, member T (testis-specific)
• H1FTMGC163222
• H1t
• histone 1, H1t
• histone cluster 1, H1t
• histone H1t
• Testicular H1 histone

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H1t is a 207 amino acid long, 22 kDA protein encoded by the gene HIST1H1T that lacks polyA tails and instead obtain palindromic termination elements. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1T is found on chromosome 5. HIST1H1T has been investigated in childhood leukemia and hemochromatosis. It is involved in granzyme-A pathway, histone modification, and PKA signaling and is known to interact with various genes such as YWHAQ, YWHAZ, ACTA2, POU5F1, and GSK3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-6