Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - HDAC8 monoclonal antibody (M01), clone 2F4 Analysis of HDAC8 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of monoclonal antibody to HDAC8 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.Lane 1: HDAC8 transfected lysate(41.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free Summary

• Immunogen: HDAC8 (AAH50433, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV
• Specificity: histone deacetylase 8
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

• EC 3.5.1.98
• HD8
• HDAC8
• HDACL1
• Histone Deacetylase 8
• histone deacetylase-like 1
• RPD3

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone Deacetylase 8/HDAC8 Antibody (H00055869-M01)(1)

Publication using H00055869-M01

• Nakayama T, Akagawa K. The transcription regulation mechanism of the syntaxin 1A gene via protein kinase A. Biochem J 2017-05-30 [PMID: 28559304]

Bioinformatics

• Gene Symbol: HDAC8
• Entrez:
  • 55869(Human)
• OMIM:
  • 300269(Human)
• Uniprot:
  • AAH50433()