Histone Deacetylase 2/HDAC2 Antibody (SD0816)

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of Histone Deacetylase 2/HDAC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate Predicted band size: 55 kDa Observed band size: 60 kDa

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Deacetylase 2/HDAC2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Hela

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Histone Deacetylase 2/HDAC2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Deacetylase 2/HDAC2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Deacetylase 2/HDAC2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (SD0816) [NBP2-67781] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Histone Deacetylase 2/HDAC2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Histone Deacetylase 2/HDAC2 Antibody (SD0816) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human Histone Deacetylase 2/HDAC2 aa 1-150/488. (SwissProt: Q92769 Human; SwissProt: P70288 Mouse; Unigene: 1797 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone Deacetylase 2/HDAC2 Antibody (SD0816)

• EC 3.5.1.98
• HD2
• HDAC2
• Histone Deacetylase 2
• RPD3
• transcriptional regulator homolog RPD3
• YAF1
• YY1-associated factor 1

Background

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC2