Histidase Antibody

Western Blot: Histidase Antibody [NBP2-27348] - analysis of His on a His tagged recombinant protein in the 1) absence and 2) presence of immunizing peptide and 3) GST tagged (non His) recombinant protein using His antibody at 2 ug/ml.

Product Discontinued

Histidase Antibody Summary

• Immunogen: This antibody was raised against His
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4ug/ml

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Histidase Antibody

• EC 4.3.1
• EC 4.3.1.3
• HIS
• histidase
• histidine ammonia-lyase
• HSTD

Background

Poly Histidine-tag is an amino acid motif which contains at least five histidine (His) residues and usually at the N- or C-terminus of a recombinant protein. His tags facilitate affinity purification of recombinant protein containing polyhistidine tags in Escherichia coli and other prokaryotic expression system. The polyhistidine-tag can also be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE) with fluorescent probes bearing metal ions. Anti-polyhistidine-tag antibodies can be useful in subcellular localization, ELISA, western blotting or other immuno-analytical methods.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAL