Histamine N-Methyltransferase/HNMT Antibody

Images

 
Western Blot: Histamine N-Methyltransferase/HNMT Antibody [NBP2-30047] - Western blot analysis of HNMT Antibody (N-term) (NBP2-30047) in Hela cell line lysates (35ug/lane). HNMT(arrow) was detected using the purified ...read more
Immunocytochemistry/ Immunofluorescence: Histamine N-Methyltransferase/HNMT Antibody [NBP2-30047] - Confocal immunofluorescent analysis of HNMT Antibody (N-term)(NBP2-30047) with Hela cell followed by Alexa Fluor ...read more
Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP2-30047] - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term) (NBP2-30047), which was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
6.64 mg/ml

Order Details

Histamine N-Methyltransferase/HNMT Antibody Summary

Immunogen
This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
Localization
Cellular Location Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNMT
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
33.295 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
6.64 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Histamine N-Methyltransferase/HNMT Antibody

  • EC 2.1.1.8
  • Histamine NMethyltransferase
  • Histamine N-Methyltransferase
  • HMT
  • HNMT
  • HNMT-S1
  • HNMT-S2

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for Histamine N-Methyltransferase/HNMT Antibody (NBP2-30047)

Discover related pathways, diseases and genes to Histamine N-Methyltransferase/HNMT Antibody (NBP2-30047). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Histamine N-Methyltransferase/HNMT Antibody (NBP2-30047)

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Bioinformatics

Gene Symbol HNMT
Uniprot