Histamine N-Methyltransferase/HNMT Antibody (RB12380)

Product Discontinued

Histamine N-Methyltransferase/HNMT Antibody (RB12380) Summary

• Immunogen: This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
• Localization: Cellular Location Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNMT
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Western Blot 1:1000
• Theoretical MW: 33.295 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 6.64 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Histamine N-Methyltransferase/HNMT Antibody (RB12380)

• EC 2.1.1.8
• Histamine NMethyltransferase
• Histamine N-Methyltransferase
• HMT
• HNMT
• HNMT-S1
• HNMT-S2

Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNMT
• Uniprot:
  • P50135(Human)