Histamine H2R Antibody

Western Blot: Histamine H2R Antibody [NBP1-78790] - (0.5ug/ml) staining of Mouse Stomach lysate (A) and (2ug/ml) Rat Small Intestine lysate (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Histamine H2R Antibody Summary

• Immunogen: Peptide with sequence C-SHNSHKTSLRLNNS, from the internal region of the protein sequence according to NP_001399939.1; NP_001010973.1.
• Specificity: This antibody is expected to recognize Mouse NP_001399939.1 iso 3, NP_001010973.1 iso 1 and Rat NP_001010973.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HRH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5-2 ug/ml
• Application Notes: WB: Approx. 50kDa band observed in Mouse Stomach and Rat Small Intestine lysates (calculated MW of 47.3kDa according to Mouse NP_001399939.1 and 45.2kDa according to Rat NP_001399940.1). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histamine H2R Antibody

• Gastric Receptor 1
• Gastric receptor I
• H2R
• HH2R
• Histamine H2 R
• histamine H2 receptor
• Histamine H2R
• histamine receptor H2
• HRH2

Background

HRH2 encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine H2R Antibody (NBP1-78790)(1)

Publication using NBP1-78790

• Nakane H, Sonobe Y, Watanabe T, Nakano K. Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation. Inflamm Res 2004-07-01 [PMID: 15241568]

Bioinformatics

• Gene Symbol: HRH2
• Entrez:
  • 3274(Human)
• Uniprot:
  • NP_001124527.1(Human)