Histamine H2R Antibody

Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Histamine H2R Antibody [NB600-812] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Rt, Mu, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Histamine H2R Antibody Summary

• Description: Novus Biologicals Goat Histamine H2R Antibody (NB600-812) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Histamine H2R Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QEEKPLKLQVWSGTE corresponding to C-Terminus according to NP_071640.1, NP_001124527.1.
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HRH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to NP_001354640.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histamine H2R Antibody

• Gastric Receptor 1
• Gastric receptor I
• H2R
• HH2R
• Histamine H2 R
• histamine H2 receptor
• Histamine H2R
• histamine receptor H2
• HRH2

Background

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine H2R Antibody (NB600-812)(2)

Publications using NB600-812

• Pal S, Gashev A, Roy D Nuclear localization of histamine receptor 2 in primary human lymphatic endothelial cells Biology open 2022-07-15 [PMID: 35776777] (ICC/IF, Rat)
• Nakane H, Sonobe Y, Watanabe T, Nakano K. Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation. Inflamm Res 2004-07-01 [PMID: 15241568]

Bioinformatics

• Gene Symbol: HRH2
• Entrez:
  • 3274(Human)
• Uniprot:
  • NP_071640.1(Human)