Histamine H1R Antibody

Immunohistochemistry: Histamine H1 R Antibody [NLS1167] - Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NLS1167] - Analysis of anti-HRH1 / Histamine H1 Receptor antibody with brain (neurons and glia) at 11 ug/ ml.

Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NLS1167] - Analysis of anti-HRH1 / Histamine H1 Receptor antibody with skeletal muscle, myocytes.

Immunohistochemistry-Paraffin: Histamine H1 R Antibody [NLS1167] - Analysis of anti-HRH1 / Histamine H1 Receptor antibody with human brain, neurons and glia.

Product Discontinued

Histamine H1R Antibody Summary

• Immunogen: Synthetic 17 amino acid peptide from 2nd extracellular domain of human HRH1 / Histamine H1 Receptor.
• Localization: Cell membrane; Multi-pass membrane protein
• Specificity: Human HRH1 / Histamine H1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HRH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 11 ug/ml
• Control:

  Histamine H1R Overexpression Lysate

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 18061827). Predicted cross-reactivity based on sequence identity: Chimpanzee (100%), Gorilla (100%), Gibbon (100%). Marmoset (94%), Orangutan (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Histamine H1R Antibody

• H1R
• H1-R
• hisH1
• Histamine H1 R
• Histamine H1R
• histamine receptor H1
• HRH1
• subclass H1

Background

The H1 Histamine Receptor has been implicated in the inflammatory cascade and allergic response. It is expressed by some inflammatory cells and by vascular smooth muscle as well as smooth muscle of other organs. Activation of this receptor expressed in the central nervous system has been shown to regulate the release of neurotransmitters and hormones. H1 receptors modulate a variety of physiological functions including arousal, anxiety, nociception, learning, and food and water intake. The histamine H1 receptor has been reported to be expressed in brain, kidney, lung, nasal cavity, placenta, skeletal and smooth muscle, synovium, and vessel. ESTs have been isolated from B-cell/lung/testis, brain, heart, liver/spleen, lung, and nerve libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histamine H1R Antibody (NLS1167)(1)

Publication using NLS1167

• Dagli M, Goksu N, Eryilmaz A et al. Expression of histamine receptors (H(1), H(2), and H(3)) in the rabbit endolymphatic sac: an immunohistochemical study. Am J Otolaryngol. 2008-01-01 [PMID: 18061827] (IHC-P, Rabbit)
  
  Details:
  Antibodies cited [IHC(paraffin), rabbit endolymphatic sac tissue]: 1. IMG-71324 (H1 receptor), Fig. 2. 2. IMG-71325 (H2 receptor), Fig. 3. 3. IMG-71328 (H3 receptor), Fig. 4.

Bioinformatics

• Gene Symbol: HRH1
• Entrez:
  • 3269(Human)