Recombinant Human HINT1 Protein

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SDS-Page: HINT1 Protein [NBC1-28612] - HINT1, 13.8 kDa (126aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human HINT1 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-126 of Human HINT1

Source: E.coli

Amino Acid Sequence: MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HINT1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
13.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human HINT1 Protein

  • Adenosine 5'-monophosphoramidase
  • EC 3.-
  • HINT
  • histidine triad nucleotide binding protein 1
  • histidine triad nucleotide-binding protein 1
  • histidine triad nucleotide-binding protein
  • PKCI1
  • PKCI-1FLJ30414
  • PRKCNH1FLJ32340
  • Protein kinase C inhibitor 1
  • Protein kinase C-interacting protein 1

Background

HINT1, also known as Histidine triad nucleotide-binding protein 1, is a member of superfamily named for a near C-terminal HXHXHXX motif (H:Histidine, X:a hydrophobic amino acid) positioned at the alpha-phosphate of nucleotide substrates. This protein hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Although it was originally thought to be a protein kinase C inhibitor and act as a haplod-insufficient tumor suppressor including spontaneous tumor formation in Hint+/- and Hint-/-, its actual physiologic function is not known. Recombinant human HINT1 protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol HINT1