HIC5/TGFB1I1 Antibody [Alexa Fluor® 488]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

HIC5/TGFB1I1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human HIC5
  Val184-Gly288
  Accession # O43294
• Specificity: Detects endogenous human HIC5 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

HIC5 (Hydrogen peroxide inducible clone 5; also ARA55) is a 50-55 kDa group III member of the LIM domain family of proteins. It is expressed primarily in smooth muscle, platelets and myoepithelium. It resides in both cytoplasm and nucleus, and performs multiple functions. It associates with focal adhesions, binds to the nuclear matrix, and serves as a coactivator for the glucocorticoid and androgen receptors. Human HIC5 is 461 amino acids (aa) in length. It contains four Leu:Asp‑rich motifs (aa 1‑215) and four LIM domains (aa 226‑461). LIM domains, either individually, or in combination, perform the majority of functions. LIM4 binds to the nuclear matrix, LIMs 3 and 4 are coactivators, and LIMs 2 and 3 bind to focal adhesions. HIC5 is induced by TGF beta and by hydrogen peroxide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.