hHR23b Antibody

Western Blot: hHR23b Antibody [H00005887-B02P] - Analysis of RAD23B expression in human liver.

Immunocytochemistry/Immunofluorescence: hHR23b Antibody [H00005887-B02P] - Analysis of purified antibody to RAD23B on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: hHR23b Antibody [H00005887-B02P] - Analysis of purified antibody to RAD23B on formalin-fixed paraffin-embedded human prostate. (antibody concentration 3 ug/ml)

Western Blot: hHR23b Antibody [H00005887-B02P] - Analysis of RAD23B expression in transfected 293T cell line by RAD23B polyclonal antibody. Lane 1: RAD23B transfected lysate(44.99 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

hHR23b Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: RAD23B (AAH20973, 1 a.a. - 409 a.a.) full-length human protein. MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGVPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED
• Specificity: RAD23B - RAD23 homolog B (S. cerevisiae),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RAD23B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and tissue lysate for WB. It has been used for IF, IHC-P, and ELISA. IP usage reported in scientific literature (PMID: 24258024).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for hHR23b Antibody

• HHR23B
• HR23BUV excision repair protein RAD23 homolog B
• P58
• RAD23 (S. cerevisiae) homolog B
• RAD23 homolog B (S. cerevisiae)
• RAD23, yeast homolog of, B
• XP-C repair complementing complex 58 kDa
• XP-C repair complementing protein
• XP-C repair-complementing complex 58 kDa protein

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hHR23b Antibody (H00005887-B02P)(1)

Publication using H00005887-B02P

• Krzeszinski JY, Choe V, Shao J et al. XPC promotes MDM2-mediated degradation of the p53 tumor suppressor. Mol. Biol. Cell 2014-01-01 [PMID: 24258024] (IP, Human)

Bioinformatics

• Gene Symbol: RAD23B
• Entrez:
  • 5887(Human)
• Uniprot:
  • AAH20973(Human)