HGF Activator Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

HGF Activator Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Pro-HGF Activator
  Gln35-Ser653
  Accession # Q9R098
• Specificity: Detects mouse Pro-HGF Activator in direct ELISAs. In direct ELISAs, less than 4% cross-reactivity with the active form of recombinant mouse (rm) HGF Activator and no cross-reactivity with rmHGF propeptide and rmHGF is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Activator Antibody [Alexa Fluor® 647]

• HGF Activator
• HGFA
• Hgfac

Background

Pro-HGF-A (Hepatocyte growth factor activator protein) is a 96 kDa member of the PA-FXII-HGFA family of enzymes. It is secreted by hepatocytes and is found in plasma at approximately 40 nM concentration. Other cells selectively reported to express HGF-A include astrocytes, endothelium, fibroblasts and macrophages. Upon activation, HGF-A is known to activate both proHGF and proMSP via proteolytic cleavage. The circulating 96 kDa form of mouse HGF-A represents an inactive 619 amino acid (aa) proform (aa 35-653). Within this proform exists one FN type II domain (aa 100-147), an EGF-like domain (aa 157-195), an FN type III domain (aa 197‑237), a second EGF-like domain (aa 238-276), a kringle domain (aa 283-364) and a functional peptidase S1 domain (aa 406-644). Activation of HGF-A typically occurs with a thrombin-mediated cleavage between Arg405-Ile406 and a KLK cleavage between Arg369-Val370. This creates a 34 kDa heterodimeric active product that contains a 2 kDa N-terminus (aa 370-405) disulfide linked to a 32 kDa C-terminus (aa 406-653). Once activated, the 34 kDa heterodimer binds to cell surface HAI‑1, rendering it unavailable to circulating HGF. When needed, this HGF-A:HAI-1 complex is released, HGF-A dissociates from HAI-1, and HGF-A is free to activate proHGF. Over aa 35-653, mouse HGF-A shares 92% and 82% aa identity with rat and human HGF-A, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.