HGD Antibody (1F1) - Azide and BSA Free

Western Blot: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1. Lane 1: HGD transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HGD rabbit polyclonal antibody.

Sandwich ELISA: HGD Antibody (1F1) [H00003081-M11] - Detection limit for recombinant GST tagged HGD is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HGD Antibody (1F1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HGD (AAH20792, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHLELPDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVLTALRRPARSSWHLRGLPMAPWHLCLNHL
• Specificity: HGD - homogentisate 1,2-dioxygenase (homogentisate oxidase) (1F1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HGD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HGD Antibody (1F1) - Azide and BSA Free

• AKU
• EC 1.13.11.5
• HGOFLJ94126
• homogentisate 1,2-dioxygenase (homogentisate oxidase)
• homogentisate 1,2-dioxygenase
• homogentisate oxidase
• Homogentisate oxygenase
• Homogentisic acid oxidase
• homogentisicase

Background

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HGD
• Entrez:
  • 3081(Human)
• Uniprot:
  • AAH20792(Human)