Recombinant Human HERC5 His Protein

SDS-Page: Recombinant Human HERC5 Protein [NBP2-51684] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human HERC5 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 681-1024 of Human HERC5
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HERC5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HERC5 His Protein

• CEB1E3 ISG15--protein ligase HERC5
• CEBP1
• cyclin-E binding protein 1
• Cyclin-E-binding protein 1
• EC 6.3.2
• EC 6.3.2.-
• HECT domain and RCC1-like domain-containing protein 5
• hect domain and RLD 5
• HECT E3 ubiquitin ligase
• probable E3 ubiquitin-protein ligase HERC5

Background

The HERC5 gene encodes for a 1,024 amino acid long, 116kDA E3 1SG15-protein ligase HERC5 protein near a cluster of HERC family genes located on chromosome 4. The protein coded for by the HERC5 gene localizes to the cytoplasm and perinuclear region and works as an interferon-induced E3 protein ligase. In doing so, it controlls ISGylation of protein targets. The HERC5 gene has been researched regarding influenza, malaria, teratoma, angelman syndrom, and carcinoma. The HERC5 gene interacts in interferon signaling, cytokine signaling in the immune system, and negative regulation of RIG-I/MDA4 signaling with interactions in NME2, CCNA2, ERCC2, PITX2, and NME1-NME2 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HERC5