HERC5 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

HERC5 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HERC5.
  
  

  Source: E. coli

  Amino Acid Sequence: SKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFA

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: HERC5
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-91985.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for HERC5 Recombinant Protein Antigen

• CEB1E3 ISG15--protein ligase HERC5
• CEBP1
• cyclin-E binding protein 1
• Cyclin-E-binding protein 1
• EC 6.3.2
• EC 6.3.2.-
• HECT domain and RCC1-like domain-containing protein 5
• hect domain and RLD 5
• HECT E3 ubiquitin ligase
• probable E3 ubiquitin-protein ligase HERC5

Background

The HERC5 gene encodes for a 1,024 amino acid long, 116kDA E3 1SG15-protein ligase HERC5 protein near a cluster of HERC family genes located on chromosome 4. The protein coded for by the HERC5 gene localizes to the cytoplasm and perinuclear region and works as an interferon-induced E3 protein ligase. In doing so, it controlls ISGylation of protein targets. The HERC5 gene has been researched regarding influenza, malaria, teratoma, angelman syndrom, and carcinoma. The HERC5 gene interacts in interferon signaling, cytokine signaling in the immune system, and negative regulation of RIG-I/MDA4 signaling with interactions in NME2, CCNA2, ERCC2, PITX2, and NME1-NME2 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HERC5