HERC3 Antibody (2C1) - Azide and BSA Free

Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01] - Analysis of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.

Sandwich ELISA: HERC3 Antibody (2C1) [H00008916-M01] - Detection limit for recombinant GST tagged HERC3 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HERC3 Antibody (2C1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HERC3 (NP_055421, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWG
• Localization: Cytoplasmic. Cytoplasmic vesicle. Note=Also found in vesicular-like structures.
• Specificity: HERC3 - hect domain and RLD 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HERC3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HERC3 Antibody (2C1) - Azide and BSA Free

• EC 6.3.2
• HECT domain and RCC1-like domain-containing protein 3
• hect domain and RLD 3
• KIAA0032EC 6.3.2.-
• probable E3 ubiquitin-protein ligase HERC3

Background

HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HERC3
• Entrez:
  • 8916(Human)
• OMIM:
  • 605200(Human)
• Uniprot:
  • NP_055421(Human)