HERC3 Antibody (2C1)

Images

 
Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01] - Analysis of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Sandwich ELISA: HERC3 Antibody (2C1) [H00008916-M01] - Detection limit for recombinant GST tagged HERC3 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HERC3 Antibody (2C1) Summary

Immunogen
HERC3 (NP_055421, 1 a.a. - 109 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWG
Localization
Cytoplasmic. Cytoplasmic vesicle. Note=Also found in vesicular-like structures.
Specificity
HERC3 - hect domain and RLD 3
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HERC3
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HERC3 Antibody (2C1)

  • EC 6.3.2
  • HECT domain and RCC1-like domain-containing protein 3
  • hect domain and RLD 3
  • KIAA0032EC 6.3.2.-
  • probable E3 ubiquitin-protein ligase HERC3

Background

HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HERC3
Entrez
OMIM
Uniprot