Hephaestin Overexpression Lysate (Native)

Images

 
Western Blot: Hephaestin Overexpression Lysate (Adult Normal) [NBL1-11506] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hephaestin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Hephaestin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215550 and based on accession number NM_138737. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hephaestin (HEPH), transcript variant 1, mRNA.
Gene
HEPH

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
136.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Hephaestin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hephaestin Overexpression Lysate (Native)

  • EC 1.16.3.1
  • hephaestin
  • KIAA0698CPL

Background

The protein encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) is the longer transcript and encodes the full-length isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-12553
Species: Hu
H00004891-M01
Species: Hu
Applications: WB, ELISA, IHC-P
NBP1-84071
Species: Hu
Applications: WB, IHC, IHC-P
MAB7509
Species: Hu
Applications: WB, IHC
NBP1-21502
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-914
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
AF4409
Species: Mu
Applications: WB
NBP1-88824
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24696
Species: Hu
Applications: IHC-P
AF3720
Species: Hu, Mu
Applications: WB, ICC
NBP2-20454
Species: Hu
Applications: WB, IHC, IHC-P
E2-616
Species: Hu

Publications for Hephaestin Lysate (NBL1-11506) (0)

There are no publications for Hephaestin Lysate (NBL1-11506).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hephaestin Lysate (NBL1-11506) (0)

There are no reviews for Hephaestin Lysate (NBL1-11506). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hephaestin Lysate (NBL1-11506) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Hephaestin Products

Bioinformatics Tool for Hephaestin Lysate (NBL1-11506)

Discover related pathways, diseases and genes to Hephaestin Lysate (NBL1-11506). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Hephaestin Lysate (NBL1-11506)

Discover more about diseases related to Hephaestin Lysate (NBL1-11506).
 

Pathways for Hephaestin Lysate (NBL1-11506)

View related products by pathway.

PTMs for Hephaestin Lysate (NBL1-11506)

Learn more about PTMs related to Hephaestin Lysate (NBL1-11506).

Blogs on Hephaestin

There are no specific blogs for Hephaestin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

UbcH5a/UBE2D1
E2-616-100

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hephaestin Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HEPH