FAQs for HE4/WFDC2 Recombinant Protein (H00010406-P01). (Showing 1 - 2 of 2 FAQs).
I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following link: www.novusbio.com/support/innovators-reward.html
We will want to use this full-length protein, but we will also want to use the protein with the GST tag removed. How can we remove the tag from this sequence?
This is a product we distribute for a Taiwanese company called Abnova. Here is a link, provided by Abnova, to the protocol for removing the GST tag from the full length protein.