HE4/WFDC2 Antibody (676013) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: IHC
• Clone: 676013
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

HE4/WFDC2 Antibody (676013) [Janelia Fluor® 525] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HE4/WFDC2
  Thr28-Phe124
  Accession # Q14508
• Specificity: Detects human HE4/WFDC2 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WFDC2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for HE4/WFDC2 Antibody (676013) [Janelia Fluor® 525]

• dJ461P17.6
• EDDM4
• Epididymal secretory protein E4
• epididymis-specific, whey-acidic protein type, four-disulfide core
• HE4
• HE4MGC57529
• Major epididymis-specific protein E4
• Putative protease inhibitor WAP5
• WAP domain containing protein HE4-V4
• WAP four-disulfide core domain 2
• WAP four-disulfide core domain protein 2
• WAP5
• WAP5epididymal protein 4
• WFDC2

Background

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HE4/WFDC2 Antibody (FAB6274JF525). (Showing 1 - 1 of 1 FAQ).

1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
   • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

Bioinformatics

• Gene Symbol: WFDC2