HDAC3 Antibody (EP462Y)

Product Discontinued

HDAC3 Antibody (EP462Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3 was used as an immunogen.
• Epitope: (N-term)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC3
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100-250
  • Immunohistochemistry-Paraffin 1:100-250
  • Western Blot 1:1000-2000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with: Mouse, Rat, Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for HDAC3 Antibody (EP462Y)

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

Histone deacetylase 3 belongs to the histone deacetylase family type 1 which actively deacetylate Histones. Currently 3 known isoforms exists (HDAC3, HDAC3A and HDAC3C) (1). HDAC3 represses transcription and binds transcription factor YY1 to form a large complex. N-Cor binds HDAC3 in a different manner than other HDACs. In addition to N-Cor, HDAC3 will form a complex with corepressor SMRT (2). HDAC3 is also known to interact with DAXX, HDAC10 and DACH1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3
• Entrez:
  • 8841(Human)
  • 15183(Mouse)
• Uniprot:
  • O15379(Human)