HCN3 Antibody (S141-28)

Images

 
Western Blot: HCN3 Antibody (S141-28) [NBP2-12896] - analysis of Rat brain membrane lysate showing detection of HCN3 protein using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28. Load: 15 ug protein. Block: 1.5% BSA ...read more
Immunohistochemistry-Paraffin: HCN3 Antibody (S141-28) [NBP2-12896] - Tissue: brain tissue. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody at ...read more
Immunohistochemistry-Paraffin: HCN3 Antibody (S141-28) [NBP2-12896] - Analysis of HCN3 in human hippocampal tissues using SMC-306. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, MiAr
Clone
S141-28
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HCN3 Antibody (S141-28) Summary

Immunogen
Fusion protein amino acids 660-779 (C terminus) of mouse HCN3
Localization
Membrane
Specificity
Detects approx 90kDa. No cross-reactivity against other HCNs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HCN3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Microarray
Application Notes
1 ug/ml of HCN3 Antibody was sufficient for detection of HCN3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HCN3 Antibody (S141-28)

  • hyperpolarization activated cyclic nucleotide-gated potassium channel 3
  • KIAA1535MGC131493
  • potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Background

1 ug/mL was sufficient for detection of HCN3 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HCN3