HCN3 Antibody (S141-28)

Western Blot: HCN3 Antibody (S141-28) [NBP2-12896] - analysis of Rat brain membrane lysate showing detection of HCN3 protein using Mouse Anti-HCN3 Monoclonal Antibody, Clone S141-28 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry-Paraffin: HCN3 Antibody (S141-28) [NBP2-12896] - Tissue: brain tissue. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN3 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Granular staining of some Purkinje cells. Staining appears to be focal.

Immunohistochemistry-Paraffin: HCN3 Antibody (S141-28) [NBP2-12896] - Analysis of HCN3 in human hippocampal tissues using SMC-306. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Product Discontinued

HCN3 Antibody (S141-28) Summary

• Immunogen: Fusion protein amino acids 660-779 (C terminus) of mouse HCN3
• Localization: Membrane
• Specificity: Detects approx 90kDa. No cross-reactivity against other HCNs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HCN3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of HCN3 Antibody was sufficient for detection of HCN3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HCN3 Antibody (S141-28)

• HCN3
• hyperpolarization activated cyclic nucleotide-gated potassium channel 3
• KIAA1535
• KIAA1535MGC131493
• potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Background

1 ug/mL was sufficient for detection of HCN3 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HCN3