HAI-2/SPINT2 Antibody (170018) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 170018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HAI-2/SPINT2 Antibody (170018) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HAI-2
  aa 28-197
  Accession # O43291
• Specificity: Detects recombinant human (rh)HAI-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhHAI-1 or rmHAI-2B.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPINT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human HAI-2 Ectodomain Antibody (Catalog # MAB1106)
  ELISA Detection: Human HAI-2 Biotinylated Antibody (Catalog # BAF1106)
  Standard: Recombinant Human HAI-2A (Catalog # 1106-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-2/SPINT2 Antibody (170018) [Unconjugated]

• HAI2
• HAI-2
• HAI-2DIAR3
• HAI2FLJ45571
• Hepatocyte growth factor activator inhibitor type 2
• Kop
• kunitz-type protease inhibitor 2
• PB
• Placental bikunin
• serine peptidase inhibitor, Kunitz type, 2
• serine protease inhibitor, Kunitz type, 2

Background

HGF Activator Inhibitor type II (HAI-2), also known as placental bikunin, is a Kunitz-type protease inhibitor. It is a type I membrane protein containing 2 Kunitz inhibitor domains in its extracellular region. In mouse, at least three isoforms of HAI-2 exist as a result of alternative splicing. Isoforms 2 and 3, also known as HAI-2B and HAI-2C, respectively, are both missing the first amino-terminal Kunitz inhibitor domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAI-2/SPINT2 Antibody (MAB1106)(2)

Publications using MAB1106

• Hwang S, Kim H, Min M, Raghunathan R, Panova I, Munshi R, Ryu B Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma. J Invest Dermatol, 2015-04-24;135(9):2283-91. 2015-04-24 [PMID: 25910030] (Western Blot, Human)
• Kongkham PN, Northcott PA, Ra YS An epigenetic genome-wide screen identifies SPINT2 as a novel tumor suppressor gene in pediatric medulloblastoma. Cancer Res., 2008-12-01;68(23):9945-53. 2008-12-01 [PMID: 19047176] (IHC-P, Human)

Bioinformatics

• Gene Symbol: SPINT2
• Uniprot:
  • O43291()