HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)

Western Blot: HGFA Inhibitor 1 Antibody (4H2) [NBP2-01969] Analysis of extracts (35ug) from 9 different cell lines by using anti-HGFA Inhibitor 1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: HGFA Inhibitor 1 Antibody (4H2) [NBP2-01969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HGFA Inhibitor 1.

Flow Cytometry: HGFA Inhibitor 1 Antibody (4H2) [NBP2-01969] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HGFA Inhibitor 1 antibody, and then analyzed by flow cytometry.

Western Blot: HGFA Inhibitor 1 Antibody (4H2) [NBP2-01969] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGFA Inhibitor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGFA Inhibitor 1.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF
• Clone: OTI4H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) Summary

• Description: Novus Biologicals Mouse HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) (NBP2-01969) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SPINT1(NP_857593) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPINT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:200

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)

• HAI
• HAI1
• HAI-1
• hepatocyte growth factor activator inhibitor 1
• Hepatocyte growth factor activator inhibitor type 1
• kunitz-type protease inhibitor 1
• MANSC2
• serine peptidase inhibitor, Kunitz type 1
• serine protease inhibitor, Kunitz type 1
• SPINT1

Background

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPINT1
• Entrez:
  • 6692(Human)