HAI-1/HGFA Inhibitor 1 Antibody (169409) [Alexa Fluor® 750]

• Applications: ELISA(Cap), ELISA(Det)
• Clone: 169409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

HAI-1/HGFA Inhibitor 1 Antibody (169409) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HAI-1
  Pro37-Glu449
  Accession # NP_003701
• Specificity: Detects human HAI-1 in ELISAs. In sandwich ELISAs, no cross-reactivity with recombinant mouse HAI-1, recombinant human (rh) TFPI, rhTFPI-2, rhAPP, rhHAI-2A, rhGASP-1, or rhGASP-2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta . The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.