GUCY2C Antibody (2543C) [Phycoerythrin]

HEK293 transfected with Human GUCY2C and eGFP were stained with eGFP and either (A) Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Monoclonal Antibody (Catalog # FAB2157P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 2543C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Phycoerythrin

GUCY2C Antibody (2543C) [Phycoerythrin] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
  Ser21-Gln430
  Accession # P25092
• Specificity: Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.005 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.05% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GUCY2C Antibody (2543C) [Phycoerythrin]

• DIAR6
• EC 4.6.1
• GCC
• GC-C
• guanylate cyclase 2C (heat stable enterotoxin receptor)
• Guanylate Cyclase C
• Guanylyl Cyclase C
• GUC2C
• GUC2CEC 4.6.1.2
• GUCY2C
• heat-stable enterotoxin receptor
• hSTAR
• Intestinal guanylate cyclase
• MUCIL
• STA Receptor
• StAR
• STARSTA receptor

Background

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
7. Gong, R. et al. (2011) Science. 333:1642.
8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for GUCY2C Antibody (FAB2157P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Panagiotis Fotakis Flow Human 02/18/2024

Summary

• Application: Flow Cytometry
• Sample Tested: T84 human colon carcinoma cell line
• Species: Human
• Lot: D102229

Bioinformatics

• Uniprot:
  • P25092()