GTF2A2 Antibody

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Western Blot: GTF2A2 Antibody [NB100-75367] - E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

GTF2A2 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 1a.a. to 109a.a. from TFIIA2(a 109a.a. long protein)
Localization
Nucleus.
Specificity
TFIIA2
Clonality
Polyclonal
Host
Chicken
Gene
GTF2A2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Positive Control
GTF2A2 Lysate (NBL1-11383)

Reactivity Notes

This antibody is reactive against Human, rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for GTF2A2 Antibody

  • General transcription factor IIA subunit 2
  • general transcription factor IIA, 2 (12kD subunit)
  • general transcription factor IIA, 2, 12kDa
  • HsT18745
  • TF2A2
  • TFIIA p12 subunit
  • TFIIA
  • TFIIA-12
  • TFIIA-gamma
  • TFIIAS
  • Transcription initiation factor IIA gamma chain
  • transcription initiation factor IIA subunit 2

Background

TFIIA (Transcription Factor IIA) is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. It interacts with TBP (the TATA binding protein). This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2A2
Entrez
Uniprot