GTF2A2 Antibody (2B9) - Azide and BSA Free

Western Blot: GTF2A2 Antibody (2B9) [H00002958-M01] - Analysis of GTF2A2 expression in transfected 293T cell line by GTF2A2 monoclonal antibody (M01), clone 2B9.Lane 1: GTF2A2 transfected lysate(12.5 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: GTF2A2 Antibody (2B9) [H00002958-M01] - Detection limit for recombinant GST tagged GTF2A2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GTF2A2 Antibody (2B9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GTF2A2 (AAH00287, 1 a.a. ~ 109 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE
• Specificity: GTF2A2 - general transcription factor IIA, 2, 12kDa (2B9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GTF2A2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GTF2A2 Antibody (2B9) - Azide and BSA Free

• General transcription factor IIA subunit 2
• general transcription factor IIA, 2 (12kD subunit)
• general transcription factor IIA, 2, 12kDa
• HsT18745
• TF2A2
• TFIIA p12 subunit
• TFIIA
• TFIIA-12
• TFIIA-gamma
• TFIIAS
• Transcription initiation factor IIA gamma chain
• transcription initiation factor IIA subunit 2

Background

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2A2
• Entrez:
  • 2958(Human)
• Uniprot:
  • AAH00287(Human)