Recombinant Human GTF2A1 His Protein

SDS-Page: Recombinant Human GTF2A1 Protein [NBP2-23074] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human GTF2A1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-274 of Human GTF2A1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANSANT NTVPKLYRSV IEDVINDVRD IFLDDGVDEQ VLMELKTLWE NKLMQSRAVD GFHSEEQQLL LQVQQQHQPQ QQQHHHHHHH QQAQPQQTVP QQAQTQQVLI PASQQATAPQ VIVPDSKLIQ HMNASNMSAA ATAATLALPA GVTPVQQILT NSGQLLQVVR AANGAQYIFQ PQQSVVLQQQ VIPQMQPGGV QAPVIQQVLA PLPGGISPQT GVIIQPQQIL FTGNKTQVIP TTVAAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GTF2A1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 32.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human GTF2A1 His Protein

• General transcription factor IIA subunit 1
• general transcription factor IIA, 1, 19/37kDa
• glucose regulated protein, 58kD pseudogene
• MGC129969
• MGC129970
• TF2A1
• TFIIA alpha, p55
• TFIIA
• TFIIA-42
• TFIIAL
• transcription initiation factor IIA subunit 1
• Transcription initiation factor TFIIA 42 kDa subunit

Background

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Recombinant human GTF2A1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GTF2A1