GTF2A1 Antibody - BSA Free

Immunohistochemistry: GTF2A1 Antibody [NBP1-46216] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- GTF2A1/TFIIA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: GTF2A1 Antibody [NBP1-46216] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-GTF2A1/TFIIA antibody used for IP at 3 mcg/mg lysate. GTF2A1/TFIIA was also immunoprecipitated by another rabbit anti-GTF2A1/TFIIA antibody, which recognizes an upstream epitope.

Product Discontinued

GTF2A1 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 100 and 150 of human Transcription Initiation Factor IIA Subunit 1 using the numbering given in entry NP_056943.1 (GeneID 2957).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GTF2A1 Antibody - BSA Free

• General transcription factor IIA subunit 1
• general transcription factor IIA, 1, 19/37kDa
• glucose regulated protein, 58kD pseudogene
• MGC129969
• MGC129970
• TF2A1
• TFIIA alpha, p55
• TFIIA
• TFIIA-42
• TFIIAL
• transcription initiation factor IIA subunit 1
• Transcription initiation factor TFIIA 42 kDa subunit

Background

GTF2A1 bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human GTF2A1 consists of three subunits of 35 kDa (alpha subunit), 19 kDa (beta subunit) and 12 kDa (gamma subunit). The alpha and beta units are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma subunit), retains native GTF2A1 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2A1