GSTM4 Overexpression Lysate

Images

 
Western Blot: GSTM4 Overexpression Lysate (Adult Normal) [NBL1-11374] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSTM4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GSTM4 Overexpression Lysate Summary

Description
Transient overexpression lysate of glutathione S-transferase mu 4 (GSTM4), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC202097 and based on accession number NM_000850. The protein contains a C-MYC/DDK Tag.
Gene
GSTM4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GSTM4 Overexpression Lysate

  • EC 2.5.1.18
  • glutathione S-alkyltransferase M4
  • glutathione S-aralkyltransferase M4
  • glutathione S-aryltransferase M4
  • glutathione S-transferase M4
  • glutathione S-transferase mu 4
  • GST class-mu 4
  • GSTM4-4
  • GST-Mu2
  • GTM4
  • GTS-Mu2
  • MGC131945
  • MGC9247
  • S-(hydroxyalkyl)glutathione lyase M4

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GSTM4 Lysate (NBL1-11374) (0)

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-11374

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Bioinformatics

Gene Symbol GSTM4