GSTM3 Antibody (20C11.H5.E11)

Images

 
SDS-Page: GSTM3 Antibody (20C11.H5.E11) [NBP2-44175] - Lane 1: Non-reduced Mouse anti-GSTM3 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker. Lane 3: Reduced Mouse anti-GSTM3 Monoclonal Antibody. Load: 1 Ug per ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP, PAGE
Clone
20C11.H5.E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

GSTM3 Antibody (20C11.H5.E11) Summary

Immunogen
This Protein A purified GSTM3 Antibody (20C11.H5.E11) was prepared by repeated immunizations in mice with GSTGSTM3 Protein (Uniprot: P21266)
Specificity
Reacts specifically with GSTM3 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GSTM3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:50000-1:200000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • SDS-Page 1:100-1:2000
  • Western Blot 1:500-1:1000
Application Notes
This product has been tested in ELISA and SDS-Page and is suitable in Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Cross reactivity from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Notes

This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for GSTM3 Antibody (20C11.H5.E11)

  • glutathione S-transferase mu 3 (brain)
  • GST5Mu-3
  • MGC3310
  • MGC3704
  • S-(hydroxyalkyl)glutathione lyase M3

Background

GSTM3, also known as Glutathione S-transferase Mu 3, is a 225 amino acid that is approx. 27 kDa; is responsible for conjugation of reduced glutathione to an extensive number of exogenous and endogenous hydrophobic electrophiles, and may be able to regulate uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Current research is being performed on over 70 diseases and disorders including laryngeal squamous cell carcinoma, pharyngitis, laryngitis, squamous cell carcinoma, laryngeal cancer, oral cancer, cataract, colorectal cancer, carcinoma, breast cancer, astrocytoma, non-Hodgkin lymphoma, malignant pleural mesothelioma, open-angle glaucoma, Hodgkin's lymphoma, lung cancer, basal cell carcinoma, adult brain tumor, and leukoplakia. This protein has been shown to have interactions with over 70 proteins including RBL2, GSTM2, PAK2, MPG, and TFE3 in pathways such as glutathione metabolism, metabolism of xenobiotics by cytochrome P450, drug metabolism - cytochrome P450, establishment of blood-nerve barrier, and response to estrogen stimulus pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GSTM3
Uniprot