Recombinant Mouse GSTA1 His Protein

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SDS-Page: GSTA1 Recombinant Protein [NBP2-52089] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

Order Details

Recombinant Mouse GSTA1 His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-223 of Mouse GSTA1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ

Details of Functionality
Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GSTA1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Mouse GSTA1 His Protein

  • EC 1.11.1.-
  • EC 2.5.1.18
  • EC 5.3.3.-
  • glutathione S-alkyltransferase A1
  • glutathione S-aryltransferase A1
  • glutathione S-transferase 2
  • glutathione S-transferase A1
  • glutathione S-transferase alpha 1
  • glutathione S-transferase Ha subunit 1
  • GST class-alpha member 1
  • GST HA subunit 1
  • GST, class alpha, 1
  • GST2
  • GSTA1
  • GSTA1-1
  • GST-epsilon
  • GTH1
  • MGC131939
  • S-(hydroxyalkyl)glutathione lyase A1

Background

GSTA1 is glutathione S-transferase which are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GSTA1