Recombinant Human GSTA1 Protein

SDS-Page: GSTA1 Protein [NBP1-30307] - GSTA1, 25.6 kDa (222aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human GSTA1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-222 of Human GSTA1
  
  

  Source: E.coli

  Amino Acid Sequence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

• Details of Functionality: Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GSTA1
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 25.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human GSTA1 Protein

• EC 1.11.1.-
• EC 2.5.1.18
• EC 5.3.3.-
• glutathione S-alkyltransferase A1
• glutathione S-aryltransferase A1
• glutathione S-transferase 2
• glutathione S-transferase A1
• glutathione S-transferase alpha 1
• glutathione S-transferase Ha subunit 1
• GST class-alpha member 1
• GST HA subunit 1
• GST, class alpha, 1
• GST2
• GSTA1
• GSTA1-1
• GST-epsilon
• GTH1
• MGC131939
• S-(hydroxyalkyl)glutathione lyase A1

Background

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for GSTA1 Recombinant Protein (NBP1-30307). (Showing 1 - 1 of 1 FAQ).

1. Your website shows two different products for GSTA1protein. The references are: - GSTA1 Protein NBP1-30307 -0.1mg; - GSTA1 Protein P3495-0.1mg. What are the biological differences between these two products that explain such a difference in price? I wish to conduct studies of enzyme activity, so I need a functional enzyme, which one should I use?
   • Either product would work as both have been validated for use in functional studies. While both products are very similar we license them from different labs therefore they are different prices.

Bioinformatics

• Gene Symbol: GSTA1