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Recombinant GST Epitope Tag Protein

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SDS-Page: Recombinant Parasite GST Epitope Tag Protein [NBC1-18537] - GST Epitope Tag, 26kDa (224aa) with a purity of 95% by SDS - PAGE
SDS-Page: Recombinant Parasite GST Epitope Tag Protein [NBC1-18537] - GST Epitope Tag, 26kDa (224aa) with a purity of 95% by SDS - PAGE

Product Details

Summary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

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Recombinant GST Epitope Tag Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-218 of Schistosoma japonicum E.coli (Bioactivity Validated) GST Epitope Tag

Source: E.coli

Amino Acid Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
Details of Functionality
Specific activity is > 20 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GSTM1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
Use in functional reported in scientific literature (PMID 26945068).
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBC1-18537 in the following applications:

  • Func
    1 publication
  • IP
    1 publication

Reactivity Notes

Schistosoma japonicum (Blood fluke)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant GST Epitope Tag Protein

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST Epitope Tag may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant GST Epitope Tag was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant GST
Epitope Tag Protein
NBC1-18537
Recombinant GST Epitope Tag Protein
Applications: Func, PAGE, Bioactivity

Publications for GST Epitope Tag Recombinant Protein (NBC1-18537)(2)

We have publications tested in 1 confirmed species: Primate.

We have publications tested in 2 applications: Func, IP.


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Bioinformatics

Gene Symbol GSTM1