Recombinant GST Epitope Tag Protein

SDS-Page: Recombinant Parasite GST Epitope Tag Protein [NBC1-18537] - GST Epitope Tag, 26kDa (224aa) with a purity of 95% by SDS - PAGE

• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant GST Epitope Tag Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-218 of Schistosoma japonicum E.coli (Bioactivity Validated) GST Epitope Tag
  
  

  Source: E.coli

  Amino Acid Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR

• Details of Functionality: Specific activity is > 20 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GSTM1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Application Notes: Use in functional reported in scientific literature (PMID 26945068).
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Schistosoma japonicum

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant GST Epitope Tag Protein

• EC 2.5.1.18
• glutathione S-alkyltransferase
• glutathione S-aralkyltransferase
• glutathione S-aryltransferase
• glutathione S-transferase M1
• glutathione S-transferase mu 1
• GST class-mu 1
• GST HB subunit 4
• GST1
• GSTM1-1
• GSTM1a-1a
• GSTM1b-1b
• GTH4
• GTM1
• HB subunit 4
• H-B
• MGC26563
• MU
• MU-1
• S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST Epitope Tag may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant GST Epitope Tag was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for GST Epitope Tag Recombinant Protein (NBC1-18537)(2)

Publications using NBC1-18537

• Di XJ, Wang YJ, Han DY et al. Grp94 Delivers gamma-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation. J. Biol. Chem. 2016-03-04 [PMID: 26945068] (Func)
• Wang Y, Ozawa A, Zaman S et al. The tumor suppressor protein menin inhibits AKT activation by regulating its cellular localization Cancer Res 2011-01-15 [PMID: 21127195] (IP, Primate)

Bioinformatics

• Gene Symbol: GSTM1