GST Epitope Tag Antibody (6G9C6) - BSA Free

Western Blot: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Analysis using GST mouse mAb against various fusion protein with GST tag.

Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:100 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).

Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:100 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).

Product Discontinued

GST Epitope Tag Antibody (6G9C6) - BSA Free Summary

• Immunogen: Purified fusion protein with GST protein tag expressed in E. Coli.
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Simple Western 1:100
  • Western Blot 1:500-1:2000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for GST Epitope Tag Antibody (6G9C6) - BSA Free

• EC 2.5.1.18
• glutathione S-alkyltransferase
• glutathione S-aralkyltransferase
• glutathione S-aryltransferase
• glutathione S-transferase M1
• glutathione S-transferase mu 1
• GST class-mu 1
• GST HB subunit 4
• GST1
• GSTM1-1
• GSTM1a-1a
• GSTM1b-1b
• GTH4
• GTM1
• HB subunit 4
• H-B
• MGC26563
• MU
• MU-1
• S-(hydroxyalkyl)glutathione lyase

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GST Epitope Tag Antibody (NB100-2196)(1)

Publication using NB100-2196

• Lee MS, Hseu YC, Lai GH et al. High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development. Microb Cell Fact. 2011-07-23 [PMID: 21781331]

Review for GST Epitope Tag Antibody (NB100-2196) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 05/05/2015

Summary

• Application: Western Blot
• Sample Tested: Over-expressed recombinant proteins in E. coli cell lysates
• Species: Other

Bioinformatics

• Gene Symbol: GSTM1