GST Epitope Tag Antibody (6G9C6)

Images

 
Western Blot: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Analysis using GST mouse mAb against various fusion protein with GST tag.
Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was ...read more
Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:100 dilution on hUbiquitn-GST (left) & ...read more
Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) ...read more
Simple Western: GST Epitope Tag Antibody (6G9B9) [NB100-2196] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:100 dilution on hVAV1-GST, E. coli (left) & ...read more

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, Simple Western, ELISA
Clone
6G9C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

GST Epitope Tag Antibody (6G9C6) Summary

Immunogen
Purified fusion protein with GST protein tag expressed in E. Coli.
Localization
Cytoplasmic
Specificity
GST (6G9B9)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSTM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • Simple Western 1:100
  • ELISA 1:10000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2196 in the following applications:

Publications
Read Publication using NB100-2196.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Purity
Protein A or G purified

Alternate Names for GST Epitope Tag Antibody (6G9C6)

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GST Epitope Tag Antibody (NB100-2196)(1)

Review for GST Epitope Tag Antibody (NB100-2196) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NB100-2196:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot GST Epitope Tag NB100-2196
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reviewed by:
Anonymous
WB Other 05/05/2015
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Summary

ApplicationWestern Blot
Sample TestedOver-expressed recombinant proteins in E. coli cell lysates
SpeciesOther

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsSee PMID: 21781331 for further details regarding the protocol.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GST Epitope Tag Antibody (NB100-2196) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
1
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2
0
1
0

Anonymous
05/05/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol GSTM1
Entrez