GSPT1 Antibody - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754] - Mouse stomach. GSPT1 stained by GSPT1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-16754] - Human breast cancer, using GSPT1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: GSPT1 Antibody [NBP2-16754] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI.
Western Blot: GSPT1 Antibody [NBP2-16754] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the ...read more
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (NBP2-16754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the ...read more
GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH ...read more
GSPT1 antibody detects GSPT1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at ...read more
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody (NBP2-16754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the ...read more
GSPT1 antibody detects GSPT1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GSPT1 stained by GSPT1 antibody (NBP2-16754) diluted at 1:500.Antigen Retrieval: Citrate buffer, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

GSPT1 Antibody - Azide and BSA Free Summary

Description
Novus Biologicals Rabbit GSPT1 Antibody (NBP2-16754) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GSPT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Recombinant protein encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSPT1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-16754.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for GSPT1 Antibody - Azide and BSA Free

  • ERF3A
  • eRF3aFLJ38048
  • ETF3A
  • eukaryotic peptide chain release factor GTP-binding subunit ERF3A
  • Eukaryotic peptide chain release factor subunit 3a
  • FLJ39067
  • G1 to S phase transition 1,551G9.2
  • G1 to S phase transition protein 1 homolog
  • GST1

Background

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSPT1 Antibody (NBP2-16754)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional GSPT1 Products

Array NBP2-16754

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Bioinformatics

Gene Symbol GSPT1