GSK-3 beta Antibody

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Product Details

Summary
Product Discontinued
View other related GSK-3 beta Primary Antibodies

Order Details


    • Catalog Number
      NBP1-02156
    • Availability
      Product Discontinued

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GSK-3 beta Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human GSK3 beta (within residues 1-100). [Swiss-Prot# P49841]
Predicted Species
Mouse (100%), Rat (100%), Sheep (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
  • Simple Western 1:40
  • Western Blot 0.5 ug/ml
Application Notes
This GSK3 beta antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 47 kDa. In ICC/IF strong cytoplasmic staining was observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
A-431 Whole Cell Lysate
GSK-3 beta Overexpression Lysate

Reactivity Notes

Human. Predicted to react with rat, mouse, zebrafish and sheep based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 beta Antibody

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline-directed serine/threonine protein kinase that was originally identified for its role in glycogen metabolism; however, subsequently it was shown to serve pleiotropic functions, including embryonic development, gene transcription, and neuronal cell function. Mammalian GSK has two isoforms encoded by distinct genes: GSK3A produces a 51-kD GSK3-alpha protein and GSK3B a 47-kD GSK3-beta protein. Phosphorylation of Tyr279/Tyr216 on GSK3alpha/beta is important for enzymatic activity, whereas inactivation of GSK3 can be achieved through phosphorylation of Ser21/Ser9 residues within the N-terminal domain on GSK3-alpha/beta, respectively. Upstream kinases PKA, AKT/PKB, PKC, p90RSK/MAPKAP and p70S6K are known to phosphorylate Ser9 of GSK3-beta. Furthermore, GSK3 is also regulated via interactions with WNT ligand and its receptor Frizzled/co-receptor LRP5/6, and this interaction releases GSK3 from a multi-protein complex formed by beta-catenin, axin, and adenomatous polyposis coli (APC), which prevents GSK3-mediated beta-catenin degradation and induces beta-catenin-dependent gene transcription. GSK3 is one of the few signaling mediators that play central roles in a diverse range of signaling pathways, including those activated by WNT, hedgehog, growth factors, cytokines, and G protein-coupled ligands. GSK3 dysregulation, particularly hyperactivation, with various pathological conditions, including diabetes mellitus, obesity, inflammation, neurological disorders, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSK3B
Entrez
Uniprot