GSK-3 beta Antibody

Images

 
Western Blot: GSK-3 beta Antibody [NBP1-02156] - Detection of GSK3B in A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody [NBP1-02156] - GSK-3 beta antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Simple Western: GSK-3 beta Antibody [NBP1-02156] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Sh, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GSK-3 beta Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human GSK3 beta (within residues 1-100). [Swiss-Prot# P49841]
Predicted Species
Mouse (100%), Rat (100%), Zebrafish (100%), Sheep (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:40
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:100
Application Notes
This GSK3 beta antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 47 kDa. In ICC/IF strong cytoplasmic staining was observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
A-431 Lysate (NB800-PC12)
GSK-3 beta Lysate (NBL1-11362)

Reactivity Notes

Human. Predicted to react with rat, mouse, zebrafish and sheep based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 beta Antibody

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline-directed serine/threonine protein kinase that was originally identified for its role in glycogen metabolism; however, subsequently it was shown to serve pleiotropic functions, including embryonic development, gene transcription, and neuronal cell function. Mammalian GSK has two isoforms encoded by distinct genes: GSK3A produces a 51-kD GSK3-alpha protein and GSK3B a 47-kD GSK3-beta protein. Phosphorylation of Tyr279/Tyr216 on GSK3alpha/beta is important for enzymatic activity, whereas inactivation of GSK3 can be achieved through phosphorylation of Ser21/Ser9 residues within the N-terminal domain on GSK3-alpha/beta, respectively. Upstream kinases PKA, AKT/PKB, PKC, p90RSK/MAPKAP and p70S6K are known to phosphorylate Ser9 of GSK3-beta. Furthermore, GSK3 is also regulated via interactions with WNT ligand and its receptor Frizzled/co-receptor LRP5/6, and this interaction releases GSK3 from a multi-protein complex formed by beta-catenin, axin, and adenomatous polyposis coli (APC), which prevents GSK3-mediated beta-catenin degradation and induces beta-catenin-dependent gene transcription. GSK3 is one of the few signaling mediators that play central roles in a diverse range of signaling pathways, including those activated by WNT, hedgehog, growth factors, cytokines, and G protein-coupled ligands. GSK3 dysregulation, particularly hyperactivation, with various pathological conditions, including diabetes mellitus, obesity, inflammation, neurological disorders, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for GSK-3 beta Antibody (NBP1-02156): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSK3B
Entrez
Uniprot

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