GSK-3 alpha [p Ser21] Antibody [Unconjugated]

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." > DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." title="Phospho-GSK3a was detected in immersion fixed paraffin-embedded sections of human breast cancer using Rabbit Anti-Human phospho-GSK3a Monoclonal Antibody (Catalog # AF4125) at 25ug/mL at 27 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder." />

Phospho-GSK3a was detected in immersion fixed paraffin-embedded sections of human breast cancer using Rabbit Anti-Human phospho-GSK3a Monoclonal Antibody (Catalog # AF4125) at 25ug/mL at 27 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

291-G1) for 15 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 10 ng/mL PMA for 20 minutes. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Phospho-GSK-3a (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3a (S21) at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > 291-G1) for 15 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 10 ng/mL PMA for 20 minutes. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Phospho-GSK-3a (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3a (S21) at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 10 ng/mL PMA for 20 minutes. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Phospho-GSK-3a (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK-3a (S21) at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # <a class=

CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="GSK‑3a was detected in immersion fixed paraffin-embedded sections of human thymus using 5 µg/mL Human/Mouse/Rat Phospho-GSK‑3a (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

GSK‑3a was detected in immersion fixed paraffin-embedded sections of human thymus using 5 µg/mL Human/Mouse/Rat Phospho-GSK‑3a (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

GSK-3 alpha [p Ser21] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human, mouse, and rat GSK-3 alpha S21 site
• Modification: p Ser21
• Specificity: Detects human, mouse, and rat GSK-3 alpha when phosphorylated at S21. This antibody does not detect phosphorylated GSK-3 beta .
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSK3A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 alpha [p Ser21] Antibody [Unconjugated]

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK3 alpha
• GSK-3 alpha
• GSK3A

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSK-3 alpha Antibody (AF4125)(1)

Publication using AF4125

• Crouthamel MC, Kahana JA, Korenchuk S, Zhang SY, Sundaresan G, Eberwein DJ, Brown KK, Kumar R Mechanism and management of AKT inhibitor-induced hyperglycemia. Clin. Cancer Res., 2009-01-01;15(1):217-25. 2009-01-01 [PMID: 19118049] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GSK3A