GRK2 Antibody - BSA Free

Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded mouse kidney using GRK2 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded rat brain using GRK2 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded human esophageal cancer using GRK2 antibody at dilution of 1:200 (40x lens).

Western Blot: GRK2 Antibody [NBP3-03731] - Analysis of extracts of various cell lines, using GRK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit GRK2 Antibody - BSA Free (NBP3-03731) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2 (NP_001610.2). LGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GRK2 Antibody - BSA Free

• ADRBK1
• adrenergic, beta, receptor kinase 1
• BARK
• BARK1
• BARK1beta-ARK-1
• Beta-ARK1
• Beta-ARK-1
• EC 2.7.11
• EC 2.7.11.15
• FLJ16718
• G-protein coupled receptor kinase 2
• GRK2
• GRK2beta-adrenergic receptor kinase 1

Background

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK2