GRASP1 Antibody

Immunohistochemistry-Paraffin: GRASP1 Antibody [NB100-1432] - staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: GRASP1 Antibody [NB100-1432] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GRASP1 Antibody Summary

• Description: Novus Biologicals Goat GRASP1 Antibody (NB100-1432) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GRASP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence LREQTTGLAAELQ-C corresponding to N-Terminus according to NP_064522.3, NP_997555.1.
• Localization: Cell Membrane (probable)
• Specificity: This antibody is expected to recognize both reported isoforms (as represented by NP_064522.3; NP_997555.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIPAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 ug/ml
• Application Notes: WB: Preliminary experiments gave no signal but low background in human brain lysates at up to 1 ug/ml.
  IHC: Paraffin embedded Human Tonsil and Brain (Cortex).
• Control:

  GRASP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRASP1 Antibody

• DKFZp434P0630
• GRASP1
• GRASP-1MGC126595
• GRIP1 associated protein 1
• GRIP1-associated protein 1
• GRIPAP1
• KIAA1167MGC126593
• MPMGp800B12492Q3

Background

GRASP1 is a neuron specific guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF) and is associated with the GRIP/AMPA receptor complex in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRASP1 Antibody (NB100-1432)(1)

Publication using NB100-1432

• Ye B, Liao D, Zhang X et al. GRASP-1: a neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 2000-06-01 [PMID: 10896157]

Bioinformatics

• Gene Symbol: GRIPAP1
• Entrez:
  • 56850(Human)
• Uniprot:
  • NP_064522(Human)