Human Granzyme K ELISA Kit (Colorimetric)

ELISA: Human Granzyme K ELISA Kit (Colorimetric) [NBP3-11793] - Samples were spiked with high concentrations of Human Granzyme K and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Granzyme K ELISA Kit (Colorimetric) [NBP3-11793] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human Granzyme K ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 6.40
• Intra-Assay: %CV < 6.31
• Spike Recovery: 88 - 110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GZMK

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Granzyme K ELISA Kit (Colorimetric)

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• Fragmentin-3
• Granzyme 3
• granzyme K (granzyme 3; tryptase II)
• granzyme K (serine protease, granzyme 3; tryptase II)
• Granzyme K
• Granzyme-3
• Granzyme-K
• GZMK
• NK-Tryp-2
• NK-Tryptase-2
• PRSS
• TRYP2
• TRYP2granzyme-3
• Tryptase II

Background

The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K (GMZK), also designated granzyme-3 or NK-tryptase-2 (NK-TRYP-2), contains one peptidase S1 domain. Granzyme K is a serine protease localizing to the granules of natural killer cells and cytotoxic T lymphocytes. It is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Granzyme K ELISA Kit (NBP3-11793)(1)

Publication using NBP3-11793

• Aubert, A;Liu, A;Kao, M;Goeres, J;Richardson, KC;Nierves, L;Jung, K;Nabai, L;Zhao, H;Orend, G;Krawetz, R;Lange, PF;Younger, A;Chan, J;Granville, DJ; Granzyme B cleaves Tenascin-C to release its C-terminal domain in rheumatoid arthritis JCI insight 2024-10-17 [PMID: 39475853]

FAQs for Granzyme K ELISA Kit (NBP3-11793). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: GZMK