Human Granzyme A ELISA Kit (Colorimetric)

ELISA: Human Granzyme A ELISA Kit (Colorimetric) [NBP3-11790] - Samples were spiked with high concentrations of Human Granzyme A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Granzyme A ELISA Kit (Colorimetric) [NBP3-11790] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human Granzyme A ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.31
• Intra-Assay: %CV < 5.38
• Spike Recovery: 85 - 105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GZMA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Granzyme A ELISA Kit (Colorimetric)

• CTL Tryptase
• CTLA3
• CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease)
• Cytotoxic T-lymphocyte proteinase 1
• EC 3.4.21
• Fragmentin-1
• granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Granzyme A
• Granzyme-1
• GZMA
• H factor
• Hanukkah factor
• HF
• HFSP
• HFSPEC 3.4.21.78

Background

The biological function of Granzyme A is unclear. It would appear that the molecule may have a role in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice are indistinguishable from normal animals in their response to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Granzyme A ELISA Kit (NBP3-11790)(1)

Publication using NBP3-11790

• Rahman MDT, Ryu S, Achangwa C et al. Temporal Dynamics of Serum Perforin and Granzyme during the Acute Phase of SARS-CoV-2 Infection Vaccines 2023-08-01 [PMID: 37631882] (ELISA)

FAQs for Granzyme A ELISA Kit (NBP3-11790). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GZMA