GPT Activity Assay Kit (Colorimetric)

GPT Activity Assay Kit (Colorimetric) [NBP3-24459] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Serum, plasma, cell samples and animal tissue
• Standard Curve Range: 1.26 - 72.3 IU/L
• Sensitivity: 1.26 IU/L

GPT Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Spectrophotometer (505 nm)
  Assay time: 100 min
• Standard Curve Range: 1.26 - 72.3 IU/L
• Sensitivity: 1.26 IU/L
• Inter-Assay: %CV < 8.2
• Intra-Assay: %CV < 4.3
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: GPT

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for GPT Activity Assay Kit (Colorimetric)

• AAT1alanine aminotransferase 1
• ALT1
• ALT1EC 2.6.1.2
• Glutamate pyruvate transaminase 1
• glutamic-alanine transaminase 1
• Glutamic--alanine transaminase 1
• glutamic-pyruvate transaminase (alanine aminotransferase)
• Glutamic--pyruvic transaminase 1
• GPT1GPT 1

Background

GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GPT