GPT Assay Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, Flow, Func, QFN
Suitable Sample Type
Plasma, Serum, Tissue Homogenates, Cell Lysates.
Sensitivity
0.006 U/mL

Order Details

GPT Assay Kit (Colorimetric) Summary

Description
Alanine Transaminase Assay Kit is a colorimetric method for assaying the activity of ALT.
Sensitivity
0.006 U/mL
Assay Type
Colorimetric
Sample Volume
20 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Assay Kit (Colorimetric)
Gene
GPT

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Functional
  • Quantification
Publications
Read Publication using KA1294.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 96-Well Cover Sheet
  2. 96-Well Plate (Colorimetric Assay)
  3. ALT Assay Buffer (10X)
  4. ALT Cofactor
  5. ALT Initiator
  6. ALT Positive Control
  7. ALT Substrate

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GPT Assay Kit (Colorimetric)

  • AAT1alanine aminotransferase 1
  • ALT1
  • ALT1EC 2.6.1.2
  • Glutamate pyruvate transaminase 1
  • glutamic-alanine transaminase 1
  • Glutamic--alanine transaminase 1
  • glutamic-pyruvate transaminase (alanine aminotransferase)
  • Glutamic--pyruvic transaminase 1
  • GPT1GPT 1

Background

Alanine transaminase (ALT), also known as alanine aminotransferase (ALAT) or serum glutamic pyruvic transaminase (sGPT), is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. ALT mediates conversion of major intermediate metabolites, catalyzing reversible transamination between alanine and alpha-ketoglutarate to form pyruvate and glutamate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for GPT Kit (KA1294)(1)

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Bioinformatics

Gene Symbol GPT