Rat GPT ELISA Kit (Colorimetric)

ELISA: Rat GPT ELISA Kit (Colorimetric) [NBP3-18697] - Standard Reference Curve

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Urine, Tissue Extract
• Standard Curve Range: 0.5 - 32 ng/mL (example only; lot dependent)
• Sensitivity: 0.27 ng/mL (example only; lot dependent)

Rat GPT ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.5 - 32 ng/mL (example only; lot dependent)
• Sensitivity: 0.27 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV%< 10.2 (example only; lot dependent)
• Intra-Assay: CV%< 4.9 (example only; lot dependent)
• Spike Recovery: 97% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GPT

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat GPT ELISA Kit (Colorimetric)

• AAT1alanine aminotransferase 1
• ALT1
• ALT1EC 2.6.1.2
• Glutamate pyruvate transaminase 1
• glutamic-alanine transaminase 1
• Glutamic--alanine transaminase 1
• glutamic-pyruvate transaminase (alanine aminotransferase)
• Glutamic--pyruvic transaminase 1
• GPT1GPT 1

Background

GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for GPT ELISA Kit (NBP3-18697). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GPT