Western Blot: GPR81 Antibody [NBP1-51956] - Analysis of GPR81 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-51956 at 0.5g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
WB: Approx. 40 kDa band observed in human liver lysates (calculated MW of 39.3 kDa band according to NP_115943.1). An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.
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