GPR81 Antibody

Western Blot: GPR81 Antibody [NBP1-51956] - Analysis of GPR81 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-51956 at 0.5g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GPR81 Antibody Summary

• Immunogen: Peptide with sequence C-QQLARQARMKKATR corresponding to internal region according to NP_115943.1.
• Epitope: C-QQLARQARMKKATR
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HCAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human liver lysates (calculated MW of 39.3 kDa band according to NP_115943.1). An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:30926749).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR81 Antibody

• FKSG80
• G protein-coupled receptor 104
• G protein-coupled receptor 81
• GPR104
• GPR81
• G-protein coupled receptor 104
• G-protein coupled receptor 81
• HCA1
• HCAR1
• LACR1
• lactate receptor 1
• TA-GPCR
• T-cell activation G protein-coupled receptor

Background

The receptor FKSG80/GPR81 is a member of the Chemokine Receptor subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR81 Antibody (NBP1-51956)(3)

Publications using NBP1-51956

• Liu X, Li S, Cui Q et Al. Activation of GPR81 by lactate drives tumour-induced cachexia Nat Metab 2024-03-18 [PMID: 38499763]
• de Castro Abrantes H, Briquet M, Schmuziger C, et al. The Lactate Receptor HCAR1 Modulates Neuronal Network Activity through the Activation of G alpha and G beta gamma Subunits J. Neurosci. 2019-06-05 [PMID: 30926749] (WB, Mouse)
• Cai TQ, Ren N, Jin L et al. Role of GPR81 in lactate-mediated reduction of adipose lipolysis. Biochem Biophys Res Commun 2008-12-19 [PMID: 18952058]

Bioinformatics

• Gene Symbol: HCAR1
• Entrez:
  • 27198(Human)
• Uniprot:
  • NP_115943.1(Human)