GPR56 Antibody [Biotin]

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Human peripheral blood lymphocytes were stained with (A) Sheep Anti-Human GPR56 Biotinylated Affinity-purified Polyclonal Antibody (Catalog # BAF4634) or (B) control antibody (Catalog # BAF020), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

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GPR56 Antibody [Biotin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GPR56
Arg26-Val342
Accession # AAP35975
Specificity
Detects human GPR56 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) GPR30, rhGPR114, rhGPR115, rhGPR124, and rhGPR125 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GPR56
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
Readout System
Publications
Read Publication using
BAF4634 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR56 Antibody [Biotin]

  • BFPP
  • EGF-TM7-like
  • G protein-coupled receptor 56,7-transmembrane protein with no EGF-like N-terminal domains-1
  • GPR56
  • Protein TM7XN1
  • TM7LN4
  • TM7LN4G-protein coupled receptor 56
  • TM7XN1
  • TM7XN1DKFZp781L1398

Background

GPR56 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) with long extracellular N-termini (1‑3). The 693 amino acid (aa) human GPR56 contains a 25 aa signal sequence, a 377 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk followed by a GPCR proteolytic cleavage site (GPS) (1, 4). Cleavage of the 60 kDa N-terminus from the 80 kDa full length form is needed for efficient cell surface expression (5, 6). While the cleaved portion may remain non-covalently associated, it has also been found in conditioned medium of cultured cells (5). Human GPR56 shares 71%, 72%, 80%, 80% and 79% aa identity with mouse, rat, canine, equine, and bovine GPR56 within the cleaved ECD. A functional splice variant lacking the GPS site and a non-functional splice variant lacking portions of the TM domains have also been described (4). A human brain developmental disorder, bilateral frontoparietal polymicrogyria, is associated with GPR56 mutations that also show impaired GPS cleavage, intracellular trafficking, and expression at the cell surface (5). GPR56 is widely distributed, with highest mRNA or expressed sequence tag expression in brain, thyroid, skin and female reproductive system (3, 4). GPR56 expression is upregulated during cell transformation and is high in melanomas, glioblastomas and astrocytomas, but downregulated in melanomas with high metastatic potential (2, 6‑8). Although the function of GPR56 is not completely known, it is clearly an adhesion protein (6‑8). Tissue transglutaminase (TG2) is one reported ligand, binding of which inhibits melanoma growth and metastasis (6). Association of GPR56 with the tetraspanin CD81 stabilizes its complex with Gaq/11 for cell signaling (9).

  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Zendman, A.J.W. et al. (1999) FEBS Lett. 446:292.
  3. Liu, M. et al. (1999) Genomics 55:296.
  4. Bjarnadottir, T.K. et al. (2007) Gene 387:38.
  5. Jin, Z. et al. (2007) Hum. Mol. Genet. 16:1972.
  6. Xu, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:9023.
  7. Shashidhar, S. et al. (2005) Oncogene 24:1673.
  8. Ke, N. et al. (2007) Mol. Cancer Ther. 6:1840.
  9. Little, K.D. et al. (2004) Mol. Biol. Cell 15:2375.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR56 Antibody (BAF4634)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol GPR56
Uniprot