GPR52 Antibody (582671) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human GPR52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR52 Monoclonal Antibody (Catalog # MAB10357) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on isotype control antibody (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 582671
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR52 Antibody (582671) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR52
  Met1-Ile361
  Accession # Q9Y2T5
• Specificity: Detects human GPR52 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR52 Antibody (582671) [Unconjugated]

• G protein-coupled receptor 52
• GPR52
• MGC111751
• probable G-protein coupled receptor 52

Background

GPR52 is a class-A orphan G-protein-coupled receptor (GPCR) that is highly expressed in the brain and represents a promising therapeutic target for the treatment of several neuropsychiatric disorders, including Huntington's disease and schizophrenia. GPR52 is a 41KDa seven-transmembrane domain protein (7TM). Mouse and human sequences are 96% identical over the full protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Y2T5()