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![Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.](http://images.novusbio.com/fullsize/GPR18-Antibody-Western-Blot-NBP2-24918-img0001.jpg "Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.")
![Immunocytochemistry/Immunofluorescence: GPR18 Antibody [NBP2-24918] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GPR18 Antibody NBP2-24918 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24918-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of a FFPE tissue section of human tonsil using 1:200 dilution of GPR18 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0003.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Daudi cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0007.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of human breast tumor tissue using an isotype control (top) and GPR18 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP2-24918-img0002.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Ntera2 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0004.jpg)
![Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on THP-1 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/GPR18-Antibody-Flow-Cytometry-NBP2-24918-img0005.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Analysis in human lymph node and pancreas tissues. Corresponding GPR18 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP1-87573-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Analysis in human lymph node and pancreas tissues. Corresponding GPR18 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP1-87573-img0011.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP1-87573-img0008.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Staining of human lymph node shows strong cytoplasmic/membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP1-87573-img0009.jpg)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP1-87573] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP1-87573-img0010.jpg)
![Immunohistochemistry: GPR18 Antibody [NBP3-14352] - Immunohistochemistry of colon, adenocarcinoma,](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-NBP3-14352-img0002.jpg "Immunohistochemistry: GPR18 Antibody [NBP3-14352] - Immunohistochemistry of colon, adenocarcinoma,")
![Immunohistochemistry-Paraffin: GPR18 Antibody [NBP3-14352] - Immunohistochemistry of formalin-fixed, paraffin-embedded human testis, seminiferous tubule, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NBP3-14352-img0001.jpg)
![Immunohistochemistry: GPR18 Antibody [NBP3-14352] - Immunohistochemistry of brain infarct neuron and glia](http://images.novusbio.com/fullsize/GPR18 Antibody-Immunohistochemistry-NBP3-14352-img0003.jpg)
![Immunohistochemistry: GPR18 Antibody [NBP3-14352] - Immunohistochemistry of Ovary, carcinoma](http://images.novusbio.com/fullsize/nbp3-14352_rabbit-gpr18-pab-10920241103117.png)
![Immunohistochemistry-Paraffin: GPR18 Antibody [NLS3953] - Analysis of anti-GPR18 antibody with human testis.](http://images.novusbio.com/fullsize/GPR18-Antibody-Immunohistochemistry-Paraffin-NLS3953-img0001.jpg "Immunohistochemistry-Paraffin: GPR18 Antibody [NLS3953] - Analysis of anti-GPR18 antibody with human testis.")
![Western Blot: GPR18 Antibody [NBP2-87522] - WB Suggested Anti-GPR18 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/GPR18-Antibody-Western-Blot-NBP2-87522-img0001.jpg "Western Blot: GPR18 Antibody [NBP2-87522] - WB Suggested Anti-GPR18 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle")
![Western Blot: GPR18 Overexpression Lysate (Adult Normal) [NBP2-10926] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR18.](http://images.novusbio.com/fullsize/GPR18-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10926-img0001.jpg "Western Blot: GPR18 Overexpression Lysate (Adult Normal) [NBP2-10926] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR18.")