GPR17 Antibody (CL10474) - Azide and BSA Free

Analysis in human cerebral cortex and kidney tissues using NBP3-44216 antibody. Corresponding GPR17 RNA-seq data are presented for the same tissues.

Staining of human kidney shows no positivity in cells in tubules as expected.

Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of oligodendrocytes.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human prostate shows no positivity in glandular cells as expected.

Analysis in human cell line U-251 MG.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL10474
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GPR17 Antibody (CL10474) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q13304, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GPR17 Antibody (CL10474) - Azide and BSA Free

• DKFZp686M18273
• G protein-coupled receptor 17
• GPR17
• G-protein coupled receptor 17
• P2Y-like Receptor
• R12
• UDP/CysLT Receptor
• uracil nucleotide/cysteinyl leukotriene receptor

Background

GPR17, a former orphan G-coupled protein is specifically activated by P2Y and CysLT endogenous ligands, leading to both adenylyl cyclase inhibition and intracellular calcium increases. In both rats and humans GPR17 expressed in the organs which suffer ischemic damage, like the brain, heart and kidneys. Studies by Ciana, et. al. have shown that inhibition of GPR17 reduces ischemic damage in a rat focal ischemia model. These results suggest GPR17 is the common molecular target mediating brain damage by nucleotides and CysLTs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR17